Objective:
To confirm the pregnancy after 20-22 days of insemination.
To reduce duration of service period.
Principle:
PGF2α in non-luteolytic dose induces the release of oxytocin from the corpus luteum which causes let-down of milk in the lactating and pregnant cows.
Procedure:
This test is performed generally 3 hours prior to the evening milking in dairy cows (18-22 days after insemination)
Place the teat cannula in the left fore-teat and leave it for milk flow from teat cistern.
When the milk flow ceases, a small dose (2.5 mg or 0.5 ml) of Dinoprost (Lutalyse) is administered intravenously through ear vein.
If the corpus luteum of pregnancy is present, alveolar milk starts to flow about one minute later.
