Gentamicin Sulphate (Anti-mastitis prep.)

Gentamicin is an aminoglycoside antibiotic. Like other aminoglycosides, the action is to inhibit bacteria protein synthesis via binding to 30S ribosome. Through this action, it causes a misreading of the genetic code and inhibits bacterial protein synthesis. Another mechanism important for gram-negative bacteria is to disrupt the cell surface biofilm, particularly on gram-negative bacteria, to produce disruption, loss of cell wall integrity, and a rapid bactericidal effect. Magnesium and calcium are important to cross-bridge adjacent lipopolysaccharide molecules. Positive-charged aminoglycosides competitively displace Ca2+ and Mg2+ and destabilize the bacteria outer membrane. Therefore, death of the bacteria is caused by a cell surface effect rather than inhibition of the ribosome. This property is not as prominent for gram-positive bacteria unless administered with a cell wall–disrupting agent such as vancomycin or a beta-lactam antibiotic.

Milk: 48 hours